Restriction enzyme: Difference between revisions
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DNA '''restriction enzymes''' (restriction endonucleases) are [[proteins]] that cut [[DNA]] at specific sequences. Each restriction enzyme is specific to a small (commonly 4-6 base pair) sequence. These enzymes can be used to cut DNA into fragments in a specific and repeatable fashion. Uses include preparation of DNA for procedures such as a [[Southern blot]]. | DNA '''restriction enzymes''' (restriction endonucleases) are [[proteins]] that cut [[DNA]] at specific sequences. Each restriction enzyme is specific to a small (commonly 4-6 base pair) sequence. These enzymes can be used to cut DNA into fragments in a specific and repeatable fashion. Uses include preparation of DNA for procedures such as a [[Southern blot]]ting or subcloning into [[plasmid vectors]]. | ||
Revision as of 12:36, 16 February 2009
DNA restriction enzymes (restriction endonucleases) are proteins that cut DNA at specific sequences. Each restriction enzyme is specific to a small (commonly 4-6 base pair) sequence. These enzymes can be used to cut DNA into fragments in a specific and repeatable fashion. Uses include preparation of DNA for procedures such as a Southern blotting or subcloning into plasmid vectors.